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M94A0282.TXT
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1994-10-08
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Document 0282
DOCN M94A0282
TI Comparison of techniques and evaluation of three commercial monoclonal
antibodies for laboratory diagnosis of varicella-zoster virus in
mucocutaneous specimens.
DT 9412
AU Perez JL; Garcia A; Niubo J; Salva J; Podzamczer D; Martin R; Service of
Microbiology, Hospital Princeps d'Espanya, Ciutat; Sanitaria i
Universitaria de Bellvitge, L'Hospitalet de; Llobregat, Barcelona,
Spain.
SO J Clin Microbiol. 1994 Jun;32(6):1610-3. Unique Identifier : AIDSLINE
MED/94358147
AB A comparison of direct antigen detection in cell scrapings with culture
techniques (tube culture and shell vial method) for diagnosis of
varicella-zoster virus (VZV) mucocutaneous infections was done in
parallel in two groups of specimens. A total of 100 specimens were from
patients with clinical diagnosis of VZV infection (group 1), and 69 were
from patients with no suspicion of VZV infection (group 2) but mainly
with herpes simplex virus infections. In addition, three commercially
available monoclonal antibodies (Whittaker, Biosoft Clone 2013, and
Ortho 3B3) directed against VZV antigens were evaluated in parallel in
the last 87 group 1 specimens. Overall, 80% of the group 1 specimens
were confirmed positive by direct detection, in comparison with 56%
positive by tube culture and/or shell vial. None of the group 2
specimens were positive for VZV by any of the methods, and none of the
monoclonal antibodies assayed reacted with any herpes simplex virus
stock strains. Antiviral therapy and the length of evolution time of
lesions affected negatively the performance of all laboratory methods,
but to a lesser extent in direct detection techniques than in culture
techniques. The Whittaker and Biosoft reagents (indirect
immunofluorescence assay) showed statistically significant differences
in sensitivity with respect to the Ortho antibody (P = 0.002 and P =
0.039, respectively; two-tailed binomial test). Direct antigen detection
is a rapid, easy-to-perform, sensitive, and specific technique and
appears to be the method of choice for laboratory confirmation of VZV
mucocutaneous infections.
DE Antibodies, Monoclonal/*IMMUNOLOGY Antibodies, Viral/*IMMUNOLOGY
Antigens, Viral/*ANALYSIS Antiviral Agents/THERAPEUTIC USE
AIDS-Related Opportunistic Infections/DIAGNOSIS/MICROBIOLOGY
Comparative Study Cross Reactions Evaluation Studies Herpes
Zoster/COMPLICATIONS/*DIAGNOSIS/DRUG THERAPY Herpesvirus 3,
Human/GROWTH & DEVELOPMENT/IMMUNOLOGY/*ISOLATION & PURIF Human Mucous
Membrane/*MICROBIOLOGY Neoplasms/COMPLICATIONS Postoperative
Complications/MICROBIOLOGY Skin/*MICROBIOLOGY Virus Cultivation
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).